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Alternatively, the stock culture organisms could be grown in a suitable liquid medium (i.e., Soybeana hundred and fifty;Casein Digest Broth or Sabouraud Dextrose Broth) plus the cells harvested by centrifugation, then washed and resuspended in sterile saline TS to acquire a microbial count of about 1 × 108 cfu per mL. [ObserveThe estimate